|
MITOME Hidemichi
Department Matsuyama University School of Clinical Pharmacy, College of Pharmaceutical Sciences Position Associate professor |
|
| Publication Date | 2010/04 |
| Type | |
| Peer Review | With peer review |
| Title | LC-NMR identification of a novel taurine-related metabolite observed in 1H NMR-based metabonomics of genetically hypertensive rats. |
| Contribution Type | |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume, Issue, Pages | 1091-1096頁 |
| Author and coauthor | Kazuki Akira, Hidemichi Mitome, Misako Imachi, Yasuo Shida, Hiroaki Miyaoka, Takao Hashimoto |
| Details | This paper describes the LC-NMR spectroscopic identification of a novel urinary endogenous metabolite responsible for the signals, which were found as major contributors to the sepn. between genetically hypertensive rats (SHRSP) and normotensive control rats (WKY) in previous NMR-based metabonomic studies. Urine samples from 26-wk-old normotensive rats were analyzed by an LC-NMR system equipped with a reversed-phase column having high retention ability for polar compds. ${1}$H NMR spectra were continuously obtained in the on-flow mode, and the retention times of the unassigned signals in question were detd. Various two-dimensional spectra were subsequently measured for the fraction contg. the unassigned signals under the stop-flow mode, which enables for a long accumulation resulting in the enhancement of signal-to-noise ratios. The candidate compd. obtained from these LC-NMR data was synthesized, and the NMR and mass spectra were compared with those of the LC-NMR fraction. The unknown metabolite was identified as succinyltaurine from these expts. together with std. addn. expts. This novel metabolite, which is characteristic of the normotensive rats, is very interesting because it is structurally related to hypotensive taurine, and not substantially detected in the genetically hypertensive rats, which excreted more taurine than the normotensive rats. The biol. and pathophysiol. significances of succinyltaurine remain to be investigated. |
| DOI | 10.1016/j.jpba.2009.11.006 |